Proteinase K, liquid formulation
€9,999.00
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SKU: P2020-125 trenzyme
Description
Proteinase K is a broad-spectrum serine protease originally isolated from the fungus Engyodontium album (formerly Tritirachinm album).
Overview
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Product Name: Proteinase K, liquid formulation
- Catalog No.: P2020-125
- RefSeqLinks: UniProt: P06873
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Synonyms: Endopeptidase K, Tritirachium alkaline proteinase
Sequence Information
- Species: Tritirachium album
- Tags: Tag-free
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Sequence (AA 106-384):
No DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR.
Product Information
- Expression Host: Pichia pastoris
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Formulation: Supplied as a 0.22 μm filtered solution in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, pH 7.2-7.4
- Format: Liquid, stored and shipped at -80°C
- Purity: > 95% as determined by SDS-PAGE
Background Information
The recombinant enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant preparation has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
Crystal structure data indicate that the enzyme belongs to the subtilisin family, which is characterized by a catalytic triad (Asp39-His69-Ser224) in the active site.
Proteinase K does not exhibit pronounced cleavage specificity, and the preferred cleavage site is the peptide bond at hydrophobic amino acids.
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DNase Residual Testing - at 37°C for 12h |
Stability testing - Proteinase K solution at 37°C for 360 days |
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